RNA

Part:BBa_K1859015

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-09)
Revision as of 04:50, 17 September 2015 by Nomuken (Talk | contribs)

the 3´side of the intron[BBa_K1332005] +complementary seqence


BBa_K1859015 can let mRNA circularize efficiently in E. coli by combining it with BBa_K1859016 .

As for details of circular mRNA, refer to ‘iGEM Gifu 2014' .


We improved the 5´side of the intron [BBa_K1332003] in iGEM2014 and made BBa_K1859015. Circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , in 2014 cloned only sequence of ribozyme involved splicing from td intron in T4 phage. However, BBa_K1859015 is cloned extra a part of intron adding the part in 2014. A part of intron involved in BBa_K1859015 has complementarity with a part of intron involved in BBa_K1859016.

You can let mRNA circularize efficiently by using BBa_K1859015 and BBa_K1859016 than using parts of 2014.

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