Coding
Si-tag1

Part:BBa_K1592007:Design

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-05)
Revision as of 04:42, 17 September 2015 by WangjieLiu (Talk | contribs) (→‎Design Notes)


LIP prepro + E. coli ribosomal protein L2 (1-60aa) + YLcwp3 Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
    Illegal XhoI site found at 374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 395


Design Notes

In order to replace more convenient, we respectively added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2 (1-60), GS linker, YLcwp3.Also we add the 6Xhis-tag between LIP2 prepro and E. coli ribosomal protein L2 (1-60) to do verification of immunofluorescence.

Source

E. coli ribosomal protein L2 was synthesized by IDT. LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62 of our lab.

References