Intermediate

Part:BBa_K299812:Experience

Designed by: Michał Lower   Group: iGEM10_Warsaw   (2010-10-13)
Revision as of 03:53, 17 September 2015 by Yunting (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K299812

User Reviews

UNIQ8ea821fa8641a8bc-partinfo-00000000-QINU

No review score entered. yunting from SPSingapore 2015

The sequence of the part was significantly different as was documented. Our team had trouble sequencing it initially as the full suffix sequence was absent, and primers that were designed based on the documented nucleotide sequence didn't work. We had to use universal primers to sequence the first 1kb, then design new primers for the next kb. Thus we had 3 staggered rounds of sequencing.


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