Coding

Part:BBa_K1640018:Design

Designed by: Meghan Cook   Group: iGEM15_Macquarie_Australia   (2015-09-10)
Revision as of 03:40, 17 September 2015 by Meghancook (Talk | contribs) (→‎Design Notes)

CHlM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

what's different between this year and last year

synthesized from a g-block from Idt

anything else check with rob

Source

Chlamydomonas reinhardtii

References

Block, M., Tewari, A., Albrieux, C., Maréchal, E. and Joyard, J. (2002). The plant S-adenosyl-l-methionine:Mg-protoporphyrin IX methyltransferase is located in both envelope and thylakoid chloroplast membranes. European Journal of Biochemistry, 269(1): 240-248. Gibson, K.D., Neuberger, A. and Tait, G.H. (1963). Studies on the biosynthesis of porphyrin and bacteriochlorophyll by Rhodopseudomonas spheroides. 4. S-Adenosylmethionine–magnesium protoporphyrin methyltransferase. Biochemical Journal, 88(2): 325-334.

Meinecke, L., Alawady, A., Schroda, M., Willows, R., Kobayashi, M., Niyogi, K., Grimm, B. and Beck, C. (2010). Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX. Plant molecular biology, 72(6): 643-658.