Part:BBa_K1819003

Rubber oxygenase A - rubber degrading enzyme

Part BBa_K1819003 corresponds to the coding sequence of rubber oxygenase A (Rox A) from Xanthomonas sp. strain 35Y (Gene bank accession code: AGT20506.1.) This 73 kDa protein is an extracellular enzyme that catalyzes oxidative C-C cleavage of poly(cis-1,4-isoprene) to a major product, 12-oxo-4,8-dimethyltrideca-4,8-diene-1-al (ODTD; C15 tri-isoprenoid), with specific activity measured as 0.3 mol min-1 per mg. Found not only in Xanthomonas sp. but also in other Gram-negative clearing zone formers, RoxA is structurally related to cytochrome c peroxidases and its function is associated to two covalently bound heme groups. Although recently characterized by Birke et al (1), the exact enzymatic mechanism of rubber degradation by RoxA has not yet been elucidated.

This protein was codon optimized and a courtesy of Professor Ph.D. Dieter Jendrossek from the University Stuttgart.

Schematic representation of BBa_K1819003 insert

Such non-usual restriction sites may serve for cloning purposes that lead to expression of recombinant protein with no additional amino acid residue(s) between its coding sequence and the fused N-terminal tag.

3D structure of RoxA generated using PyMOL software (PDB code 4B2N).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 91
  Illegal BamHI site found at 433
  Illegal BamHI site found at 1171
  Illegal BamHI site found at 1189
  Illegal XhoI site found at 782
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 736
  Illegal NgoMIV site found at 826
  Illegal NgoMIV site found at 1003
  Illegal NgoMIV site found at 1111
  Illegal NgoMIV site found at 1115
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 362
  Illegal SapI.rc site found at 1448