Part:BBa_K1742001
Entertoxin LTB
The heat-labile enterotoxin beta subunit is an important pathogenic factor derived from the enterotoxigenic Escherichia coli and other pathogens. It causes diarrheal diseases responsible for more than 650 million cases, resulting in almost one million deaths every year [1].
Biology and Usage
The LTB gene was domesticated and standardized as a GoldenBraid part and cloned into the pUPD2 entry vector. The CDS was subsequently assembled with the constitutive promoter P35S from Cauliflower Mosaic Virus, and its terminator (T35S). The transcriptional unit was then introduced into Nicotiana benthamiana by Agrobacterium-mediated transformation and the expression levels of LTB was detected with a Western Blot analysis employing an anti-6xHystidine antibody.
Figure 1. Immunoblot assay of the expression levels of LTB protein in N. benthamiana leaves.
References
1. Kang TJ., Han SC., Jank MO., Kang KH., Jang YS., Yang MS. (2004). Enhanced expression of beta subunit of Escherichia coli heat-labile enterotoxin in Tobacco by optimization of coding sequence. Applied Biochem. and Biotech. 117: 175-187
2. Mendoza SR., Soria RL., Revilla RL., Fierros LM., Alpuche AG. (2008). Ingestion of transgenic carrots expressing the Escherichia coli heat-labile enterotoxin beta subunit protects mice against cholera toxin challenge
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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