Part:BBa_K1678002

Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase. Coding sequence has been codon optimized for Lactobacillus plantarum. Protein is expressed by a L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019). The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141). The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). The expressed protein catalyses two steps in the riboflavin pathway.