![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1678001
Designed by: Barthelemy Caron Group: iGEM15_Paris_Bettencourt (2015-09-16)
Pyrimidine reductase/pyrimidine deaminase cassette RibD
Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase. Coding sequence has been codon optimized for Lactobacillus plantarum. Protein is expressed by the strong L.plantarum synthetic promoter BBa_K1678004. The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141) The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). The expressed protein catalyses two steps in the riboflavin pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 20
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1262
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Categories
Parameters
None |