Regulatory

Part:BBa_K1723005

Designed by: Emilie Cuillery   Group: iGEM15_EPF_Lausanne   (2015-09-15)
Revision as of 21:50, 16 September 2015 by E.Cuillery (Talk | contribs)

PAM rich URS J23117Alt promoter

<b/>PAM rich URS J23117Alt is a new fully synthetic promoter obtained by mutation of PAM rich URS J23117 promoter (BBa_K1723001)</b>. It acts the exactly same way as the PAM rich URS J23117 promoter but is targeted by others specific sgRNAS. See the design section for more information about its creation. It has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: X0 (BBa_K1723006), X4 (BBa_K1723007), or X35 (BBa_K1723008). dCas9 can only bind in presence of a PAM sequence.

Discover all the parts that can work with this one:

http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

EPFL_Lausanne_promoter.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 277
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 167
    Illegal XhoI site found at 195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None