Part:BBa_K1694004

Single-chain variable fragment (Anti-EGFR)

Introduction: Single Chain Variable Fragment of Cetuximab

we selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab and named it Anti-EGFR

scFv (Single Chain Variable Fragment)

scFv(single-chain variable fragment) is a fusion protein that consist of variable region of heavy chain(VH) and light chain(VL) of antibody, and connected by a short linker. Here we use the amino acid sequence of GGSSRSSSSGGGGSGGGG as a linker to connect VH and VL.

Features of scFv:
1.specificity:the property of specificity of antibody to antigen has being maintained
2.can be simply produced or displayed by bacteria such as E. coli
3.

Cetuximab

We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that regulate the cell division and cell apoptosis.
EGFR is characterized by an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic domain containing the tyrosine kinase region followed by a carboxyl-terminal tail with tyrosine autophosphorylation sites. Mutations in the gene encoding EGFR that lead to overexpression of this protein will lead to cells proliferate uncontrollably.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]