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Part:BBa_K1679010:Design

Designed by: Cun Wei   Group: iGEM15_OUC-China   (2015-09-08)
Revision as of 19:37, 16 September 2015 by C.Leung (Talk | contribs) (→‎References)

promoter+RNA thermometer+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 700
    Illegal AgeI site found at 812
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a special RBS to make the device temperature-sensitive.When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded.Thus the ribosome can't translated RFP.And when the temperature rises above 42°C,the secondary structure of the RBS after transform would be unfolded and produce RFP.However,according to our experience,it didn't appear red when the temperature rises above 42°C.

Source

BBa_J23106

BBa_K115001

BBa_E1010

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.