Regulatory

Part:BBa_K1723001

Designed by: Emilie Cuillery   Group: iGEM15_EPF_Lausanne   (2015-09-08)
Revision as of 19:03, 16 September 2015 by E.Cuillery (Talk | contribs)

PAM rich URS j23117 promoter


PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: Z0 (BBa_K1723002), Z35 (BBa_K1723003), or Z4 (BBa_K1723004). dCas9 can only bind if its target sequence is preceded PAM sequence.

Discover all the parts that can work with this one:

http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

EPFL_Lausanne_promoter.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None