Part:BBa_K1660001

rMpL

This part encodes a nematotoxic lectin from the parasol mushroom( Macrolepiota procera) under the control of pBAD promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  Illegal XhoI site found at 151
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 208
  Illegal AgeI site found at 304
  Illegal AgeI site found at 519
  
• 1000
  COMPATIBLE WITH RFC[1000]

Background

rMpL is a typical kind of β-trefoil folded lectin, consisting of α-,β-;s and γ- repeats. The sequence of gene rMpL is 582bp long,we build this part by linking a pBAD promoter(BBa_K206000) and RBS(BBa_B0034) in the upstream region, of course we did

restriction endonuclease and SDS-PAGE to festifytg thep expression of rMpl.

Whats'more,we also designed toxicity test for nematodes,the results will be showed in the following parts.