Composite

Part:BBa_K1685011

Designed by: ITB_Indonesia 2015   Group: iGEM15_ITB_INDONESIA   (2015-09-16)
Revision as of 18:33, 16 September 2015 by Registry (Talk | contribs)

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Rhamnolipid production

This part encodes two enzymes, rhlA and rhlB (BBa_K653000), for biosynthesis of rhamnolipid. The enzymes are under T7 promoter with medium strengh and a strong ribosomal binding site (BBa_K613010), and stopped with a double terminator (BBa_B0015).

The promoter had the second highest efficiency as shown by EPFL team 2011 (https://parts.igem.org/Part:BBa_K613010:Experience).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 225
    Illegal BamHI site found at 785
    Illegal XhoI site found at 961
    Illegal XhoI site found at 2247
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 1861
    Illegal NgoMIV site found at 1974
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 450
    Illegal BsaI site found at 1490
    Illegal BsaI.rc site found at 634


[edit]
Categories
//chassis/prokaryote/ecoli
//function/biosynthesis
Parameters
positive_regulatorsIPTG, lactose