Composite

Part:BBa_K1648051:Design

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-05-31)
Revision as of 15:21, 16 September 2015 by Leoseak2 (Talk | contribs) (→‎Source)

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PfOprF with HoxKGZ flanking sequences


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 838
    Illegal BamHI site found at 1624
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 751


Design Notes

A BamHI site was included in the middle of the flanking sequence for linearizing the plasmid DNA before transformation. (After cutting with BamHI the 5' sequence in front of HoxKGZ would be at the beginning and the CDS of HoxKGZ would be at the end of the sequence so an insertion would be occur right in front of the CDS of HoxKGZ.)

Source

J13002 and K1172501 was get from the distribution kit from IGEM part registry. The HoxKGZ flanking sequence was obtained by PCR from the Azotobacter vinelandii strain DJ genome.

References