Coding

Part:BBa_K1739003:Design

Designed by: Natalie Sloan   Group: iGEM15_Kent   (2015-09-15)
Revision as of 13:42, 16 September 2015 by NSloan (Talk | contribs)

Sequence coding for Envirowire: Sup35NM with N-terminal CsgAss and C-terminal cytb562


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 718


Design Notes

The part has been sequence optimised.


Source

It comes from a genomic sequence of Saccharomyces cerevisiae (bakers yeast).

References