DNA

Part:BBa_K1614010

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-16)
Revision as of 12:33, 16 September 2015 by Sholderbach (Talk | contribs)

His Tag II AptaBody (A5)

Notice: Functional DNA

This part is a sequence of a functional ssDNA. It is only active as single-stranded DNA. It can not be cloned into a plasmid. For use order it as a DNA oligo.


His Tag II AptaBody is a sequence consisting of a His-Tag aptamer and the HRP-mimicking DNAzyme for use in Western Blot applications. Between the His Tag aptamer and the HRP mimicking DNAzyme a 5x poly-A spacer is placed ensuring the functionality for ligand binding and peroxidase activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None