Part:BBa_K1767004

P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter

We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that is activated over time.

The presented part is the first and the second module of our designed genetic circuit but without AiiA which consist of a constitutive promoter (I14032) driving expression of LuxR followed by a Lux promoter (R0062) , driving expression of TetR.

We built this BioBrick to test the degradation of AHL by AiiA and how AiiA affects the system.

You can use this BioBrick to produce TetR for a longer period compared to BioBrick BBa_K176003 as it lack the AiiA, which is an enzyme that degrades AHL hence AHL degrades slower. Hence the gene that will be cloned downstream of the TetR promoter will be expressed later in time. The scheme below explains how this BioBrick works :

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 987