Coding

Part:BBa_M31022:Design

Designed by: bryan hernandez   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-28)
Revision as of 08:48, 28 February 2007 by Registry (Talk | contribs)


Gene II (RBS X.1 silent mutant)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1234
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

I wanted to weaken RBS X and preserve II AA sequence.

II: gca ttt gag ggg gat tca X RBS: gca ttt gag ggg gat tca AA seq: ALA PHE GLU GLY ASP SER 

                       -10^

X.1 RBS region: gcg ttc gaa ggc gac tcg New AA seq: ALA PHE GLU GLY ASP SER



Source

gene II

===References===