Reporter
Part:BBa_I13001
Designed by: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG Group: Antiquity (2004-06-28)
RBS B0030 w/ YFP (-LVA) TT
B0030. E0030. B0015
Experience
Glasgow iGEM 2011
This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:
Shenzhen_SFLS (IGEM 2015)
We expressed this part in E.coli using pbad promoter and J23116 promoter.
From left to right: J23116/pbad(0μM L-Arabinose)/1.0μM/2.0μM/4.0μM(best in the 5 concentrations we usd)/8.0μM File:QQ图片20150210175650.jpeg.jpg File:QQ图片20150210175655.jpeg.jpg
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/reporter/ret
emission | yellow |
excitation | |
tag | None |