Part:BBa_K1679007

promoter+RNA thermometer+RFP

This is a device containing a promoter (BBa_J23101),a RBS (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.In theory,When the temperature rises above 42°C,It can produce RFP.However,according to our experimental,It can't appear red.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 700
  Illegal AgeI site found at 812
  
• 1000
  COMPATIBLE WITH RFC[1000]