Device
Part:BBa_K1679007
Designed by: Cun Wei Group: iGEM15_OUC-China (2015-09-07)
This is a device containing a promoter (BBa_J23101),a RBS (BBa_K115001) and a RFP coding sequence (BBa_E0010) on the pSB1C3, which can be easily transformed into E.coli.In theory,When temperature rise above 42°C,It can produce RFP.However,according to our experimental,It can't appear red.
promoter+RNA thermometer+RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 700
Illegal AgeI site found at 812 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
chassis | DH5α |
emission | 607 |
excitation | 584 |