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Composite

Part:BBa_K1632013

Designed by: Riku Shinohara   Group: iGEM15_Tokyo_Tech   (2015-08-30)
Revision as of 09:20, 16 September 2015 by JunKawamura (Talk | contribs)

PBAD/araC_rbs_fimE(wild-type)

FimE is Fim recombinase.
With the expression of FimE, the fim switch (example BBa_K1632000, BBa_K1632004 etc...) only inverts from the ON state to OFF state.
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//awards/part_collection/2015
Parameters
None