Composite

Part:BBa_K1604025

Designed by: Turcato Alessandro   Group: iGEM15_UNITN-Trento   (2015-09-12)
Revision as of 07:59, 16 September 2015 by Eli (Talk | contribs)

araC-pBAD + proteorhodopsin + j23100 + blh + araC-pBAD + β-Carotene

This is the complete device for the use of proteorhodopsin, a light-powered proton pump that needs retinal as cofactor, without providing exogenous retinal.

Usage and Biology

This device contains the five genes necessary for retinal biosynthesis and the gene for the proteorhodopsin[1] [2] . In details it contains the four genes responsible of βcarotene production (ctrEIBY) under the control of araC-pBAD. The blh gene is under a costitutive promoter of the Anderson family (J23100) and encodes for the β-carotene 15-15 dioxygenase that catalyzes the cleavage of a single molecule of β-carotene into two molecules of retinal. Proteorhodopsin, taht is under the control of araC-pBAD, is a light-powered proton pump that needs retinal as cofactor. PR creates a proton motive force across the membrane that can subsequently power cellular processes, such as ATP synthesis.

  1. Walter, Jessica M., Derek Greenfield, Carlos Bustamante, and Jan Liphardt. "Light-powering Escherichia Coli with Proteorhodopsin." Proceedings of the National Academy of Sciences 104 (2007): 2408-2412.

  2. Martinez, A., A. S. Bradley, J. R. Waldbauer, R. E. Summons, and E. F. Delong. "Proteorhodopsin Photosystem Gene Expression Enables Photophosphorylation in a Heterologous Host" Proceedings of the National Academy of Sciences 104.13 (2007): 5590-595. Web.

    3. Sequence and Features BBa_K1604025 SequenceAndFeatures

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