DNA

Part:BBa_K1732000

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-03)
Revision as of 04:39, 16 September 2015 by Li.kenn (Talk | contribs)

J23100-PelB-GlucHCO-B0015

J23100 Promoter with a RBS (BBa_S04423), PelB Leader sequence, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia princeps luciferase to be transcribed and translated using a strong promoter. Luminescence can be expressed by reacting the luciferase product with coelenterazine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]


DONNA1.jpg

[edit]
Categories
//promoter
//proteindomain/localization
//rbs/prokaryote/constitutive/constitutive
Parameters
None