Composite

Part:BBa_K1682016:Design

Designed by: iGEM15_HKUST-Rice   Group: iGEM15_HKUST-Rice   (2015-09-15)
Revision as of 03:49, 16 September 2015 by Rsakata (Talk | contribs) (→‎Design Notes)

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IPTG induced RFP reporter system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1084
    Illegal NheI site found at 1107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A strong constiuitive promoter was used to drive the expression of Lacl, this may had lead to a lower dynamic range of the system in response to IPTG (1), which lead to difficulty in RFP output measurement. The dynamic range and sensitivity possibly be increased by choice of a weaker constituitive promoter.

1. Wang, B., Barahona, M., & Buck, M. (2015). Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities. Nucleic Acids Research, 43(3), 1955-1964

Source

Biobrick

References