Composite

Part:BBa_K1682017:Design

Designed by: iGEM15_HKUST-Rice   Group: iGEM15_HKUST-Rice   (2015-09-15)
Revision as of 02:36, 16 September 2015 by Rsakata (Talk | contribs) (→‎Design Notes)


ParaBAD-GFP-Plac-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3185
    Illegal NheI site found at 3208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4386
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2882
    Illegal AgeI site found at 2994
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880
    Illegal SapI site found at 961


Design Notes

In the subpart BBa_K1682016, a strong constiuitive promoter was used to drive the expression of Lacl, this may had lead to a lower dynamic range of the system in response to IPTG (1), which lead to difficulty in RFP output measurement.


1. Wang, B., Barahona, M., & Buck, M. (2015). Amplification of small molecule-inducible gene expression via tuning of intracellular receptor densities. Nucleic acids research, 43(3), 1955-1964

Source

Biobrick parts

References