Deleted
Sample Discontinued
Experience: Issues
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Plasmid

Part:BBa_I52001:Design

Designed by: Reshma Shetty   Group:   (2007-01-29)
Revision as of 19:50, 29 January 2007 by Registry (Talk | contribs)


ccdB and nonfunctional pUC19 derived high copy origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 323


Design Notes

This part is a composite part of BBa_P1016 and BBa_I50020. It is designed to be placed in the multiple cloning site of BioBricks vectors. Vectors with this part in the multiple cloning site will be selected against during BioBricks assembly because BBa_P1016 is lethal to most cell strains. Also, BBa_I50020 enables the vector to be propagated at high copy regardless of the other origins on the vector.


Source

ccdB and pUC19

References