Composite

Part:BBa_K1688001

Designed by: Biosurfers   Group: iGEM15_Uppsala   (2015-09-15)
Revision as of 17:06, 15 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Mono-rhamnolipid genes

The genes responsible for producing the mono-rhamnolipids (biosurfactant) are called RhlA and RhlB. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene (BBa_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688001 is a composite part of BBa_K1688002 and BBa_K1688003.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
    Illegal BamHI site found at 647
    Illegal XhoI site found at 823
    Illegal XhoI site found at 2137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1030
    Illegal NgoMIV site found at 1751
    Illegal NgoMIV site found at 1864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312
    Illegal BsaI site found at 1380
    Illegal BsaI.rc site found at 496


[edit]
Categories
Parameters
None