Coding
fliC_MCS

Part:BBa_K1617000

Designed by: Johann Bauerfeind   Group: iGEM15_Berlin   (2015-09-15)
Revision as of 16:29, 15 September 2015 by Registry (Talk | contribs)

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Modular Flagellin - FliC_MCS

Contructing a multimodular Flagellin

1 Theory

1.1 Aim

Design of a functional flaggelin variant which does not contain the variable d3 flagellin domain anymore but instead carries a polylinker region (MCS). This polylinker region is the product of an inserted multi cloning side in place of the endogeneous d3 coding sequence.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 628
    Illegal BamHI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 583
    Illegal AgeI site found at 634
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None