Regulatory

Part:BBa_K1675025:Design

Designed by: Jing Yang   Group: iGEM15_BIT-China   (2015-09-15)
Revision as of 15:45, 15 September 2015 by Registry (Talk | contribs)

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P-atp2 mutant 399-B0034-lacz alpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is an EcoRI site exists in the P-atp2 sequence, so we mutate it to change it.


Source

Error prone PCR of P-atp2, then overlap extension of synthetic oligonucleotides.

The natural P-atp2 is from Corynebacterium glutamicum.

Lacz alpha is from https://parts.igem.org/wiki/index.php?title=Part:BBa_I732006


References