Part:BBa_K1648004

Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane

Construct Map for K1648004

Consist of six fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.

Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamXY, mamGFDC and mms6 operons except the mamC operon is designed for homologous recombination.

Part containing this template (K1648002) together with another part containing mamC (K1648XXX) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2759
  Illegal XbaI site found at 2765
  Illegal SpeI site found at 1
  Illegal PstI site found at 7
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2759
  Illegal SpeI site found at 1
  Illegal PstI site found at 7
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2759
  Illegal XhoI site found at 1162
  Illegal XhoI site found at 2188
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2759
  Illegal XbaI site found at 2765
  Illegal SpeI site found at 1
  Illegal PstI site found at 7
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2759
  Illegal XbaI site found at 2765
  Illegal SpeI site found at 1
  Illegal PstI site found at 7
  Illegal NgoMIV site found at 2546
  Illegal NgoMIV site found at 2636
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 2611