Device

Part:BBa_K1587006

Designed by: Alexandre Le Scornet   Group: iGEM15_Toulouse   (2015-09-14)
Revision as of 13:58, 15 September 2015 by JohnSmith (Talk | contribs)

pompC and lacI box regulated cI expression

Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes.

TLSE-Circadian.JPG

Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
chassisE. coli