Reporter

Part:BBa_K1679003

Designed by: Qikai Qin   Group: iGEM15_OUC-China   (2015-09-15)
Revision as of 12:48, 15 September 2015 by Registry (Talk | contribs)

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GFP Reporter

This is a device containing a promoter (BBa_J23117),a RBS (BBa_B0034), a GFP coding sequence (BBa_E0040) and two terminators (BBa_B0010, BBa_B0012) on the pSB1C3, which can be easily transformed into E.coli and produce GFP (mut3b). This is a GFP expression device, which can express GFP (mut3b) without any induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


[edit]
Categories
//classic/reporter
//function/reporter
//function/reporter/fluorescence
Parameters
chassisE. coli K-12 DH5-alpha
emission511nm
excitation501nm
function