Device

Part:BBa_K1587004:Design

Designed by: Alexandre Le Scornet   Group: iGEM15_Toulouse   (2015-09-14)
Revision as of 12:44, 15 September 2015 by JohnSmith (Talk | contribs)

Butyrate synthesis pathway regulated by constitutive promoter p(Bla)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 36
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1717
    Illegal BamHI site found at 3634
    Illegal XhoI site found at 1407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 332
    Illegal AgeI site found at 1254
    Illegal AgeI site found at 2900
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1752
    Illegal BsaI.rc site found at 2169
    Illegal BsaI.rc site found at 3237
    Illegal SapI site found at 123
    Illegal SapI.rc site found at 3249


Design Notes

p(Bla) is followed by NheI restriction site and ccr is followed by XhoI restriction site


Source

Sequence are from Clostridium acetobutylicum : - hbd http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2831733&to=2832581&strand=true - crt http://www.ncbi.nlm.nih.gov/nuccore/NC_003030.1?report=fasta&from=2835666&to=2836451&strand=true

Sequence are from E. coli - atoB http://www.ncbi.nlm.nih.gov/nuccore/NC_020990.1?report=fasta&from=6738960&to=6740186&strand=true - tesB http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=fasta&from=474301&to=475161&strand=true

Sequence is from Streptomyces caulinus : - ccr

The strong RBS and Terminator were used according to respectively BBa_B0030 and BBa_B1006

References