Composite

Part:BBa_K1767001

Designed by: iGEM Technion HS   Group: iGEM15_Technion_HS_Israel   (2015-09-15)
Revision as of 12:42, 15 September 2015 by Registry (Talk | contribs)

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P(Lac)IQ RBS Aiia RBS LuxR term term luxpR

We designed and built a kill switch mechanism that is based on the activation of a lethal gene downstream of an inducible promoter that is activated over time. The presented part is the first module of our designed genetic circuit (BBa_K1767003). In order to characterize and understand each module of the genetic circuit we designed two composite parts that allow us to verify the (1) the degradation rate of AHL by AiiA and (2) induction of the pLux promoter by LuxR in presence of AHL with and without co-expression of AiiA. (1) The here presented BioBrick consists of a constitutive promoter driving expression of AiiA and LuxR followed by a Lux promoter which drives expression of a fluorescent reporter (here: yellow fluorescent protein). For a schematic overview see the figure below


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1761


[edit]
Categories
//biosafety/kill_switch
Parameters
None