Regulatory

Part:BBa_K1723001:Design

Designed by: Emilie Cuillery   Group: iGEM15_EPF_Lausanne   (2015-09-08)
Revision as of 11:54, 15 September 2015 by E.Cuillery (Talk | contribs) (→‎References)


PAM rich URS j23117 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PAM rich sequence was a bit shortened comparing to the original promoter in pWJ89 plasmid as it contained one RFC10 restriction site but all the binding sites remains intact.


Source

This promoter was shipped to us by Dr David Bikard, from the pasteur institute, in the pWJ89 plasmid. [1]

References

[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.