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Part:BBa_K1723002

Designed by: Emilie Cuillery   Group: iGEM15_EPF_Lausanne   (2015-09-15)
Revision as of 11:10, 15 September 2015 by Registry (Talk | contribs)

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sgRNA Z0 expressing cassette

This part is expressing the sgRNA (single guide RNA) Z0 that can bind to dCas9-ω; (BBa_K1723000). The complex was designed to bind the PAM rich URS J23117 promoter (BBa_K1723001) to repress the production of the target gene linked to the promoter. This part is a part of a gene regulation system built with dCas9-ω;. with the parts sgRNAZ4 expressing cassette (BBa_K1723003) and gRNA Z35 expressing cassette (BBa_K1723004). This sgRNA coding sequence was biobricked with the promoter pBAD and its terminator, the one we used for our experiments, as its coding sequence has to start the first base after the promoter and so it couldn't be used with the standard biobrick system so the promoter is provided already. It is possible to PCR out the part to put it under another promoter, but he terminator is specific and it is recommended to keep it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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