Translational_Unit
Part:BBa_K1724001
Designed by: Yanrui Ye Group: iGEM15_SCUT (2015-09-05)
RBS+CsgA
The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.
The quantification data of MerR/CadA operon can be seen in BBa_K1724000
Sequence and Features
[edit]
Categories
Parameters
None |