Part:BBa_K1724001

RBS+CsgA

The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.[1]That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.

The quantification data of MerR/CadA operon can be seen in BBa_K194001 ====Reference==== 1.Hakkila KM, Nikander PA, Junttila SM, Lamminmaki UJ, Virta MP: Cd-Specific Mutants of Mercury-Sensing Regulatory Protein MerR, Generated by Directed Evolution. Applied and environmental microbiology 2011, 77(17):6215-6224. Sequence and Features BBa_K1724001 SequenceAndFeatures