Device

Part:BBa_K1631024

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-14)
Revision as of 03:19, 15 September 2015 by Registry (Talk | contribs)

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Colicin-E3 cassette (Strong)

Colicin-E3, its immunity protein, and colicin lysis protein is constitutively expressed strong.(under BBa_?J23101) When this device works, Colicin-E3 will be released to media, and E. coli strains which does not have immunity protein will be killed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 696
    Illegal AgeI site found at 68
    Illegal AgeI site found at 179
    Illegal AgeI site found at 448
    Illegal AgeI site found at 1714
    Illegal AgeI site found at 1819
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/celldeath
//function/cellsignalling
Parameters
None