Coding
AsLOV2pep

Part:BBa_K1742000:Design

Designed by: Ruben Escriba Piera   Group: iGEM15_Valencia_UPV   (2015-09-03)
Revision as of 18:51, 14 September 2015 by Ruben (Talk | contribs) (→‎Design Notes)


Avena sativa LOV2 domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LOV2 domain was fused to a small peptide epitope to the C-terminus of the J-alpha helix (-KAVDTWV). Depending on the nature of this peptide tag, its ligand ePDZ interacts with different interaction affinities. The AsLOVpep domain also contains a linker 'GGSGGS-' in the N-terminus

Source

The AsLOV2 fused to a peptide epitope was synthesized according to the second LOV domain of Avena sativa phototropin 1 (UniprotKB 049003)

References