Device
Part:BBa_K1587005:Design
Designed by: Louise GODY Group: iGEM15_Toulouse (2015-09-14)
Butyrate synthesis pathway (BBa_1587004) without ccr gene regulated by constitutive promoter p(Bla) TLS_Butyrate_pathway_without_ccr.PNG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 36
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 352
Illegal BamHI site found at 2269
Illegal XhoI site found at 42 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1535
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 387
Illegal BsaI.rc site found at 804
Illegal BsaI.rc site found at 1872
Illegal SapI.rc site found at 1884
Design Notes
There are two restriction sites of Nhe1 and Xho between p(Bla) and RBS sequence.
Source
We did the assembly of several sequences from ncbi, and hbd and crt genes which came originally from Clostridium acetobutylicum were optimized to work in E.coli.