Regulatory
Part:BBa_K1707013
Designed by: Sylvie Lautru, Audrey Moatti Group: iGEM15_Paris_Saclay (2015-09-14)
Revision as of 14:39, 14 September 2015 by SylvieL (Talk | contribs) (Created page with "The translation of the thermosensitive cI repressor (cI857) is placed under the control of an RNA thermometer. This construction allows the controlled repression of the expressio...")
The translation of the thermosensitive cI repressor (cI857) is placed under the control of an RNA thermometer. This construction allows the controlled repression of the expression of a gene A with a cI promoter within the 30-35°C range of temperature. Below 30°C, the secondary structure formed by the RNA thermometer in the mRNA prevents the translation of cI857, A is therefore expressed. Above 30°C, the RNA thermometer is unfolded, allowing the translation of cI857. Under 35°, this later is properly folded and active, repressing the expression of A. Above 35°C, cI857 is under an inactive form and A is expressed.
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