Regulatory

Part:BBa_K1707013:Design

Designed by: Sylvie Lautru, Audrey Moatti   Group: iGEM15_Paris_Saclay   (2015-09-14)
Revision as of 13:57, 14 September 2015 by Registry (Talk | contribs)

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cI857 under the control of RNA thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During the design, we had to make sure that the RBS included in the RNA thermometer would be at a correct location in front of the cI857 gene when we will assemble the parts. Luckily, the RNA thermometer part (K115017) had been designed to allow the assembly with protein coding part.


Source

This part is the result of the assembly of existing parts.

References