Coding

Part:BBa_K1597003

Designed by: Marieke Mulder   Group: iGEM15_Groningen   (2015-09-14)
Revision as of 13:22, 14 September 2015 by Marieke.groningen (Talk | contribs)

bslA, forms hydrophobic surface layer on biofilm

To make our biofilm more robust and hydrophobic we we have focussed on bslA. bslA forms a hydrophobic surface layer on the biofilm of Bacillus subtilis. This layer is essential for the water repelling properties of the biofilm of the Bacillus subtilis. The BslA protein itself is a cell wall protein with amphiphilic properties which self-assembles into polymers at the air-water interface in vitro. For the expression of bslA, expression of epsABCDEFGHIJKLMNO and tapA-sipW-tasA operons are required. Without bslA the microstructure of the biofilm is smoothened and the repellency against water and organic solvents is reduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None