RNA

Part:BBa_K1859001:Experience

Designed by: Wataru Fukuda   Group: iGEM15_Gifu   (2015-09-07)
Revision as of 13:08, 14 September 2015 by Nomuken (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1859001

User Reviews

UNIQ8417484a0d76004f-partinfo-00000000-QINU UNIQ8417484a0d76004f-partinfo-00000001-QINU

You attach this part to 5 'sides of any parts. In addition, you couple mRNA circularization device (3'side) in 3 'sides. When this device is expressed in E.coli, Curcular mRNA including the linker is made. The efficiency that mRNA is circularized changes by sequence of the mRNA. We let mRNA of RFP circularize and produce a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.