Coding
c2 P22

Part:BBa_C0053

Designed by: Maia Mahoney   Group: Antiquity   (2003-01-31)
Revision as of 17:36, 12 March 2007 by Scmohr (Talk | contribs)

c2 repressor from Salmonella phage P22 (+LVA)

The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degradation.


Usage and Biology

P22 c2 is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type c2 to contain an LVA tag,

and two TAA stop codons.


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 166


[edit]
Categories
//cds
//cds/transcriptionalregulator/repressor
//function/regulation/transcriptional
Parameters
directionForward
functionRep
ligands?
proteinC2 P22
swissproP69202
tagLVA