Composite

Part:BBa_K1638039

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-09-14)
Revision as of 11:53, 14 September 2015 by Registry (Talk | contribs)

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Hfq fused to T25 domain of CyaA with cAMP-induced RFP reporter

The Hfq RNA-binding protein fused to the T25-domain of the adenylate cyclase, CyaA, from Bordetella pertussis. This construct can be used in the two-hybrid screening of protein-protein interactions between Hfq and another x protein fused to the T18 domain. If a random set of peptide aptamers is conjugated to the T18-domain, one can screen for specific peptide aptamers against Hfq.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1851
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None