Part:BBa_K1864001:Experience
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Applications of BBa_K1864001
We built yeast which contains two parts. The first part is CSBV-RdRP (Part:BBa_K1864000) and the second part is T7 RNA polymerase. Meanwhile, IPTG could induce the operation of T7 RNA polymerase. Therefore,we tested the induction results of different IPTG concentration. And we got the data which
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IPTG inductive effect
We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
The inductive effect of different concentration IPTG
We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
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The inductive effect of different time length
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
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